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HPLC determination of 4-amino-5-chloro-N-[(1-ethylimidazolin-2-yl)methyl]-2-methoxybenzamide and its metabolite in biological fluids. Applications to pharmacokinetic studies.
Summary
Type:
HPLC
Application #:
105050
Detection:
fluorescence, excitation 270nm, emission 370nm
Conditions:
eluent: acetonitrile - 1g/l citric acid + 1ml triethylamine pH 4.5 (10:90)
flow rate 1.8μl/min
Authors:
Bressolle, F. et al.
Source:
J. Pharm. Sci. 79 (1990) 534–539
Details
Legend: Fig. 1. Chromatogram of an extract of human plasma spiked with (left) 5ng/ml 1 + 10ng/ml metabolite (2), (middle) 10ng/ml 1 + 25ng/ml 2, (right) 25ng/ml 1 + 50ng/ml 2; 3 = 4-amino-5-(methylaminosul
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